Aim: This paper investigates vaccine storage and distribution in Kushtia, Bangladesh. Planning and executing the distribution of vaccines and maintaining their quality plays a vital role in preventing the spread of infectious diseases. This study aims to identify the vaccine distribution processes, vaccine handling, and systems management processes to achieve the goal of ensuring the successful operation of the vaccine supply chain system and to help determine if promoting the outsourcing of vaccine cold chain in Kushtia is a viable strategy. Materials and Methods: Survey data was collected from both public and private institutions. The research involved a total of 403 respondents (371 males and 22 females). The study integrates spatial analysis using static maps and web maps and contains data on the most commonly used vaccine, the most needed vaccine, the age group who receives the highest number of vaccinations, vaccine distribution information, vaccine logistics information, inventory information, and finally the problem of vaccine distribution. Results and Conclusion: The study identified that there is a need for proper system storage and distribution in the rural area of Bangladesh and offers recommendations to improve the existing status.